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2 edition of Platelet subpopulation heterogeneity found in the catalog.

Platelet subpopulation heterogeneity

Geoffrey Lacey

Platelet subpopulation heterogeneity

biochemical and functional studies.

by Geoffrey Lacey

  • 139 Want to read
  • 9 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Birmingham, Dept of Biochemistry.

ID Numbers
Open LibraryOL13802493M

  A distinct platelet subpopulation (smaller platelets) with lower FSC but relatively similar CD41 expression appeared in activated samples concomitant with an increase in the number of platelet. Tbx15 is a member of the T-box gene family of mesodermal developmental genes. We have recently shown that Tbx15 plays a critical role in the formation and metabolic programming of glycolytic myofibers in skeletal muscle. Tbx15 is also differentially expressed among white adipose tissue (WAT) in different body depots. In the current study, using three independent methods, we show that even.

The shape and parameters of platelet size distributions were studied in 50 normal persons and 97 patients in order to test the proposed thesis that platelet size heterogeneity results mainly from aging in the circulation. This thesis was contradicted (1) by size distributions of age-homogeneous, new . Battle is a practical and sometimes lasting way of solving man's problems. It relies on the strength of the combatants and ignores the truth of the dispute. Discussion face to face can dissolve attitu.

The physiological heterogeneity of platelets leads to diverse responses and the formation of discrete subpopulations upon platelet stimulation. Procoagulant platelets are an example of such subpopulations, a key characteristic of which is exposure either of the anionic aminophospholipid phosphatidylserine (PS) or of tissue factor on the. Pellet platelets by centrifugation at x g for 15–20 min at room temperature (with no brake applied). Discard the supernatant. Rinse the platelet pellet with platelet wash buffer (without resuspension in order to avoid unnecessary platelet activation) by gently adding wash buffer and removing it slowly with a pipette. Repeat once.


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Platelet subpopulation heterogeneity by Geoffrey Lacey Download PDF EPUB FB2

Although lacking a nucleus, platelets are increasingly recognized not only for their complexity, but also for their diversity. Some 50 years ago platelet subpopulations were characterized by size and density, and these characteristics were thought to reflect platelet aging.

Since, our knowledge of platelet heterogeneity has grown to recognize that differences in platelet biochemistry and Cited by: 5. Objective— Previous work has shown that platelets stimulated with the combination of thrombin and convulxin, a glycoprotein VI agonist, develop 2 populations with different levels of α-granule factor V bound to the platelet surface.

To evaluate whether this phenomenon is restricted to α-granule components or is a feature that can be generalized to other coagulation factors, we studied the. Hence, in this mini-review, we summarize the current knowledge of platelet subpopulation diversity focusing on their physical, biochemical, and functional heterogeneity.

In addition, we review how platelet subpopulations may change between health and disease and how differences among platelets may influence response to anti-platelet by: 5. High dimensional analysis revealed the presence of a platelet subpopulation with significantly higher levels of surface expression of activated GPIIb-IIIa and P-selectin suggesting this subpopulation may play a greater role in thrombus formation than other platelet subpopulations.

Thus, analysis by mass cytometry of platelet heterogeneity and. A continuous flow electrophoresis procedure has been developed to study platelet subpopulation heterogeneity with separations based upon surface elect Cited by:   Since cyclic AMP is known to play an important role in the regulation of platelet function (3) and protein phosphorylation during the signal transducing process (26,27), it is reasonable to suggest that the lower aggregability of our dense-platelet subpopulation (SP III) is related to higher levels of cyclic Platelet subpopulation heterogeneity book and cyclic AMP.

The biochemical basis for the functional heterogeneity of human blood platelets was investigated in terms of protein phosphorylation, cytoplasmic calcium ([Ca 2+] i), the ratio of 46 and 50 kDa vasodilatorstimulated protein (VASP), and GTP-binding proteins (G-proteins).Platelets were fractionated by density.

Fibrin coat. Fibrin-coated platelets are a subpopulation of phosphatidylserine-exposing platelets that bind fibrin via transglutaminase activity and activated integrin αIIbβ3. Circulating platelets consist of subpopulations with different age, maturation state and size.

In this review, we address the association between platelet size and platelet function and summarize the current knowledge on platelet subpopulations including reticulated platelets, procoagulant platelets and platelets exposing signals to mediate their clearance.

Clinical heterogeneity is the type of heterogeneity most related to subgroup and subpopulation issues and therefore of major interest to clinical and policy-level decision-makers. Methodological heterogeneity reflects differences in study design and conduct, including risk of bias, across studies in the systematic review [ 7 ].

Platelets -winner of a Highly Commended BMA Medical Book Award for Internal Medicine - is the definitive current source of state-of-the-art knowledge about platelets and covers the entire field of platelet biology, pathophysiology, and clinical ly there has been a rapid expansion of knowledge in both basic biology and the clinical approach to platelet-related diseases.

Generation and characterization of procoagulant platelet subpopulations. Platelets were isolated from human venous blood collected from healthy non-medicated individuals as detailed previously.

20 Washed platelets (1×10 8 /mL) were activated with thrombin (50nM) in the presence of RGDS (1mM) to prevent platelet aggregation. Alternatively, platelets were activated with a combination of low. Flow cytometric platelet analyses.

Washed human or mouse platelets (5×10 7 /mL) were pre-incubated with indicated inhibitors or Me 2 SO vehicle for 10 min, and stimulated in the presence of 2 mM CaCl the activations, mM Gly-Pro-Arg-Pro (GPRP) was added to prevent formation of large fibrin fibers.

19 Platelet sub-populations were distinguished by probing with Rhod-A14 (10 μg/mL. Platelets: with special reference to platelet density subpopulations, stable coronary heart Recent research concerning platelet subpopulation 22 CD40 Ligand Soluble CD40 Ligand 23 the significance of platelet heterogeneity remains unclear and stimulates to further research.

In particular, future work must involve more patients. Request PDF | The role of RNA uptake in platelet heterogeneity | The role of platelets in regulating vascular homeostasis has expanded beyond mediation of haemostasis and thrombosis.

The discovery. Battle is a practical and sometimes lasting way of solving man's problems. It relies on the strength of the combatants and ignores the truth of the dispute.

Discussion face to face can dissolve attitudes which have incorrectly determined judgements. The most striking example of this that I know is.

Methods. A linear Percoll™ gradient covering the density span to kg/L was used to separate whole blood platelets from type 2 diabetic subjects (n = 12) into 17 density gradient contained theophylline, prostaglandin E 1 and EDTA to prevent platelet activation in vitro.A multi-colour flow cytometer was employed for analysing the characteristics mentioned above.

The formation of this subpopulation was shown to be a stochastic process determined by the small number of activated PAR1 receptors and by heterogeneity in the number of ion pumps. These results demonstrate how a gradual increase of the activation degree can be converted into a stepped response hierarchy ultimately leading to formation of two.

To further interrogate platelet RNA processes, we utilized sorting and RNA sequencing to develop platelet subpopulation transcriptome profiles. We found that platelet heterogeneity extends to the platelet transcriptome: distinct RNA profiles exist dependent on platelet size.

We hypothesized that this RNA heterogeneity is the result of RNA. Therefore, an assay quantifying the subpopulation of platelets binding factor Xa may be useful in determining an individual's ability to generate thrombin. Indeed, in a washed platelet system, the percent of activated platelets binding factor Xa could be correlated in concurrent kinetic experiments with thrombin generation (r=, n=18) (P.

A subpopulation of platelets responds to thrombin- or SFLLRN-stimulation with binding sites for factor IXa. J Biol Chem. ; – Crossref Medline Google Scholar; 7 Kempton CL, Hoffman M, Roberts HR, Monroe DM.

Platelet heterogeneity: variation in coagulation complexes on platelet subpopulations. Arterioscler Thromb Vasc Biol.Get this from a library! Platelet Heterogeneity: Biology and Pathology. [John Martin; Anthony Trowbridge] -- Battle is a practical and sometimes lasting way of solving man's problems.

It relies on the strength of the combatants and ignores the truth of the dispute. Discussion face to face can dissolve.Measurement. Platelet concentration is measured either manually using a hemocytometer, or by placing blood in an automated platelet analyzer using electrical impedance, such as a Coulter counter.

The normal range (99% of population analyzed) for platelets in healthy Caucasians istoper cubic millimeter (a mm 3 equals a microliter). or – × 10 9 per liter.